The Combination of PD-L1 and CTLA-4 Suppression Significantly Decreased the Expression Levels of Cancer Stem Cell Factors in the Pancreatic Cancer Cell Line

Background: It is thought that a limited number of aberrant cancer stem cells (CSCs), which encourage carcinogenesis, tumor metastasis, and treatment resistance, are the cause pancreatic cancer, disease with high mortality rate. Increasing evidence shows CSCs use immunosuppressive properties to avoid immune system identification. In Pancreatic therapeutic consequences relationship between expression checkpoints such as programmed death receptor ligand-1 (PD-L1) cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) presence poorly known. As result, in present investigation, we examined how levels were affected by PD-L1 CTLA-4 knockdown using specific siRNA. Methods: Independently together, siRNA transfected into MIA PaCa-2 cells. RNA extraction cDNA synthesis then performed. Finally, qRT-PCR, gene markers, including Nanog, CD133, CD44, Oct-4, groups measured. Results: cell line, siRNA-mediated inhibition decreased CSC factors. Moreover, combined suppression these two stemness-related significantly Oct-4 compared separately. Conclusion: Considering combination factors, this approach may be regarded an effective therapy for cancer.